In this free webinar, learn about rabbit antibody discovery using the Beacon single-cell cloning process. The featured speaker will present the scientific background of the workflow and give an overview of the characteristics of the antibodies discovered in this case study.
TORONTO, Nov. 27, 2023 /PRNewswire-PRWeb/ -- In this webinar, the featured speaker will discuss the rapid single B-cell cloning workflow that ChemPartner is using to discover rabbit antibodies. After a quick primer on the technical equipment they are using (Beacon), they will use an internal anti-CD28 (cluster of differentiation 28) rabbit antibody discovery case study to go over the individual steps of the rabbit workflow. Attendees will get an understanding of the Beacon system, the scientific background of the workflow, as well as an overview of the characteristics of the antibodies discovered in this case study.
CD28 is a costimulatory receptor for T-cell activation, and CD28 binding antibodies are in clinical development to block or promote T-cell activation. CD28-specific binding domains are also used in bi- or tri-specific antibody formats to generate T-cell-engaging antibodies. They set out to generate CD28-specific monoclonal antibodies (mAbs) from rabbits using a single B-cell cloning workflow on the Beacon system.
The Beacon system enables rapid antibody discovery using a function-first approach. Antibody-secreting cells (ASCs) are isolated from immunized animals and placed individually into nanowells (pens) of a Beacon chip. Due to the small size of the pens, the secreted antibody concentration reaches µg/ml levels within minutes, and the functional properties of the antibodies can be tested in sequential or multiplexed bead-based assays with a fluorescent readout. Typical assay duration is 30-60 minutes.
In summary, the rabbit antibody discovery workflow on the Beacon enabled rapid screening of a large number of ASCs and identification of antigen-specific antibodies within four days of obtaining peripheral blood mononuclear cells (PBMCs) from immunized rabbits. cDNA generation and export happened on the day of the Beacon run, and cDNA amplification added another day to the workflow. The generation of constructs and expression of antibodies for further characterization was completed in less than one month. Overall, the method was robust and substantially reduced the hands-on time for rabbit antibody discovery, especially compared to the traditional hybridoma approach.
Join this webinar to gain insights into ChemPartner's innovative single B-cell cloning workflow and the groundbreaking Beacon system.
Join Andreas Loos, PhD, Director Biologics Discovery ChemPartner, for the live webinar on Monday, December 11, 2023, at 1pm EST (10am PST).
For more information, or to register for this event, visit A Rapid Single Cell Cloning Workflow for Antibody Discovery from Rabbits: A Case Study.
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